A collection of /possibly useful/ tools to speed up work.
Design partially overlapped primers. Input sequence and desired mutations if any and see melting temperatures of different parts of the primers. Adjust primer length as needed.
Search protein sequences (or any other sequence) for occurrence of motifs, amino acid, protease sites, etc.
This tool can also fetch sequences based on a UniProt ID, and search RegEx expressions.
Use this tool to fit binding data. Currently, fitting anisotropy data for a titration to determine binding affinity is supported.
For additional information about the how to determine binding affinity see: How to determine binding affinity.
Last updated: 2018 July 19