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Determine Binding Kd

Determining binding affinity (KD) of proteins

Want to fit your binding data? Online fitting tool available here.

Selected links to publications addressing methods for determining binding affinities.
At present most of these refer to anisotropy methods.

BEST ANISOTROPY MANUAL
(Although this manual is very comprehensive, there is a single error in the anisotropy fitting equation)

Article: A Guide to Simple and Informative Binding Assays

Article: Analysis of protein-ligand interactions by fluorescence polarization

Class: Cy dyes, lab handouts about fluorescence anisotropy 

Additional links addressing practical aspects of fluorescence anisotropy including basic methods and error analysis.

Anisotropy
  • Basics of FRET Microscopy
  • DNA-Protein Interactions
  • Fluorescence anisotropy / polarization IUPAC methods and reference
  • Fluorescence anisotropy error / uncertainty analysis
  • Fluorescence Spectroscopy and Microscopy : Methods and Protocols
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