Determining binding affinity (KD) of proteins
Want to fit your binding data? Online fitting tool available here.
Selected links to publications addressing methods for determining binding affinities.
At present most of these refer to anisotropy methods.
BEST ANISOTROPY MANUAL
(Although this manual is very comprehensive, there is a single error in the anisotropy fitting equation)
Additional links addressing practical aspects of fluorescence anisotropy including basic methods and error analysis.